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human il 1 β  (Proteintech)


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    Proteintech human il 1 β
    Human Il 1 β, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+1+%CE%B2+elisa+kit/Human+IL-1+beta+ELISA+Kit/pm41560397-407-34-37
    Average 95 stars, based on 89 article reviews
    human il 1 β - by Bioz Stars, 2026-10
    95/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Satellite glial GPR37L1 and its ligand maresin 1 regulate potassium channel signaling and pain homeostasis
    Article Snippet: .. Mouse IL-1β levels were measured using a mouse IL-1β ELISA Kit (R&D, Cat No. MLB00C, Minneapolis, MN), Human IL1β were detected using a human IL-1 β ELISA Kit (Proteintech, Rosemont, IL; Cat No. KE00021) according to the manufacturer’s instructions. ..



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    B. bifidum CIP-01 protects intestinal barrier from HiAlc Kpn W14-induced toxic damage in vitro and in vivo. A and B The effects of HiAlc Kpn W14 and B. bifidum CIP-01 on NCM460 cells are analyzed by CCK-8 assay ( A ) and the detection of lactate dehydrogenase concentration. C – G Immunofluorescence microscopy analysis (20X) in NCM460 cells on apoptosis by a Annexin V-PE probe (red) ( C ), on ROS by ROS tracker (red) ( D ), on calcium influx by a fluorescent probe Fluo-4 AM (green) ( E ), on mitochondrial morphology with Mito-tracker (green/red) ( F ), and on ER with ER-tracker (red) ( G ), and nucleus is counterstained with Hoechst33342 (blue), n = 5; scale bars are not shown for clarity. H The concentration of inflammatory cytokines TNF- α and IL-1 β in the supernatant are detected by ELISA kits, n = 5. All the NCM460 cells are treated with HiAlc Kpn W14 (MOI = 10) alone, co-treated with W14 and supernatant of B. bifidum CIP-01 culture medium (50 μL ), as well as EtOH (500 μM), respectively, for 24 h, n = 6. (I) ZO-1, MUC-2, and Occludin proteins in intestinal tissues of mice (n = 3) are evaluated by IHC (40X). (J) The serum level of DAO from SPF mice fed with HiAlc Kpn W14, B. bifidum CIP-01, EtOH and a chow diet for 4 and 6 weeks. All data are presented as the mean ± SD. ns, P > 0.05, * P < 0.05 , ** P < 0.01 , *** P < 0.005, **** P < 0.001 . P value was assessed by two-way ANOVA

    Journal: Journal of Gastroenterology

    Article Title: Bifidobacterium bifidum CIP-01 attenuates metabolic dysfunction-associated steatotic liver disease induced by high-alcohol-producing Klebsiella pneumoniae

    doi: 10.1007/s00535-025-02332-x

    Figure Lengend Snippet: B. bifidum CIP-01 protects intestinal barrier from HiAlc Kpn W14-induced toxic damage in vitro and in vivo. A and B The effects of HiAlc Kpn W14 and B. bifidum CIP-01 on NCM460 cells are analyzed by CCK-8 assay ( A ) and the detection of lactate dehydrogenase concentration. C – G Immunofluorescence microscopy analysis (20X) in NCM460 cells on apoptosis by a Annexin V-PE probe (red) ( C ), on ROS by ROS tracker (red) ( D ), on calcium influx by a fluorescent probe Fluo-4 AM (green) ( E ), on mitochondrial morphology with Mito-tracker (green/red) ( F ), and on ER with ER-tracker (red) ( G ), and nucleus is counterstained with Hoechst33342 (blue), n = 5; scale bars are not shown for clarity. H The concentration of inflammatory cytokines TNF- α and IL-1 β in the supernatant are detected by ELISA kits, n = 5. All the NCM460 cells are treated with HiAlc Kpn W14 (MOI = 10) alone, co-treated with W14 and supernatant of B. bifidum CIP-01 culture medium (50 μL ), as well as EtOH (500 μM), respectively, for 24 h, n = 6. (I) ZO-1, MUC-2, and Occludin proteins in intestinal tissues of mice (n = 3) are evaluated by IHC (40X). (J) The serum level of DAO from SPF mice fed with HiAlc Kpn W14, B. bifidum CIP-01, EtOH and a chow diet for 4 and 6 weeks. All data are presented as the mean ± SD. ns, P > 0.05, * P < 0.05 , ** P < 0.01 , *** P < 0.005, **** P < 0.001 . P value was assessed by two-way ANOVA

    Article Snippet: Mouse serum and cell culture supernatants were collected to measure the release of TNF- α (E-EL-H0109/E-EL-M3063, Elabscience®), IL-1 β (E-EL-H0253, Elabscience®) with ELISA kits according to the manufacturer’s instructions, and the absorbance was then measured at 450 nm using a microplate reader.

    Techniques: In Vitro, In Vivo, CCK-8 Assay, Concentration Assay, Immunofluorescence, Microscopy, Enzyme-linked Immunosorbent Assay